Cited as = species name adopted by the researcher that performed the cytogenetic analysis; 2n = diploid number of males and in parenthesis, diploid number of females; n = haploid number;

3n = triploid number; 4n = tetraploid number; SCS = sex chromosome system; T = telocentric; A = acrocentric; St = subtelocentric; Sm = submetacentric; M = metacentric; H = holocentric;

S = supernumerary chromosome; (?) = dubious description; (*) = designation of the species name not recorded in the taxonomic database.

Valid name	Cited as	2n	SCS	Chromosomal morphology	Collection site	Bibliography
Acanthoscurria gomesiana Mello-Leitão, 1923	----	43-46	----	M, Sm, St, A	Brazil	Oliveira, 1998 apud Řezáč et al., 2006
Aphonopelma hentzi (Girard, 1852)	Dugesiella hentzi	44	X1X2	----	----	Painter, 1914
Avicularia minatrix Pocock, 1903	----	78	X1X2X3X4	52M/Sm+4St+18A+X1Sm+X2M+X3M+X4A	----	Kořínková & Král, 2013
Brachypelma albopilosum Valerio, 1980	Brachypelma albopilosa	74	----	----	----	Vítková et al., 2005
B. albopilosum Valerio, 1980	----	74	X1X2X3X4	64M/Sm+6St+X1X2X3X4M	Costa Rica	Král et al., 2013
Chaetopelma olivaceum (C.L. Koch, 1841)	Chaetopelma anatolicum	(16)	----	----	Turkey	Akan et al., 2005
Grammostola rosea (Walckenaer, 1837)	----	72	X1X2	64M+4Sm+2St+X1M+X2Sm	Chile	Král et al., 2013
Holothele cf. longipes	----	73	X1X2X3	66M/Sm+4A/T+X1X2X3M/Sm	Venezuela	Král et al., 2013
Idiothele mira Gallon, 2010	----	25	X	18M+2Sm+2St+2A+XM	South Africa	Král et al., 2013
Ischnocolus jickelii L. Koch, 1875	----	85	X1X2X3	70M+12Sm+X1X2X3M/Sm	Yemen	Král et al., 2013
Pelinobius muticus Karsch, 1885	----	67	X1X2X3	46A+18M/Sm+X1M+X2St+X3A	Kenya	Král et al., 2013
Poecilotheria formosa Pocock, 1899	----	110	X1X2X3X4	30M+18Sm+18St+40A+X1X2X3M+X4Sm	India	Král et al., 2011
Psalmopoeus cambridgei Pocock, 1895	----	84	X1X2	64M/Sm+18A+X1X2M	Trinidad	Král et al., 2013
Pterinochilus murinus Pocock, 1897	----	43	X	36M+6Sm+XM	Tanzania	Král et al., 2013
Vitalius dubius (Mello-Leitão, 1923)	----	74-81	----	M, Sm, St, A	Brazil	Oliveira, 1998 apud Řezáč et al., 2006
Vitalius roseus (Mello-Leitão, 1923)	----	48	----	St, A	Brazil	Lucas et al., 1993 apud Řezáč et al., 2006
Vitalius sorocabae (Mello-Leitão, 1923)	----	48	----	St, A	Brazil	Lucas et al., 1993 apud Řezáč et al., 2006
Vitalius wacketi (Mello-Leitão, 1923)	----	48	----	St, A	Brazil	Lucas et al., 1993 apud Řezáč et al., 2006
Vitalius sp.	----	48	----	M, Sm, St, A	Brazil	Oliveira, 1998 apud Řezáč et al., 2006