Analysis of Chromosome Morphology - LEVAN



LEVAN (Sakamoto & Zacaro, 2009) is a ROImanager like plugin which enables the first imput for karyotype description and idiogram elaboration using relative lenght data. LEVAN is an analysis plugin for ImageJ, an open-source multi-plataform software for Image Processing and Analysis in Java developed by W. Rasband at National Institutes of Health, NIH, USA (Rasband, 1997-2012; Abramoff et al., 2004).

LEVAN classifies the chromosomal morphology of meiotic (bivalents and univalents) or mitotic metaphase chromosomes in digital images. The morphological criterion of the plugin is a modification of the criteria described by Levan et al. (1964) and Green & Sessions (1991).

The first two requirements to use LEVAN are download and install ImageJ. Then, download and install LEVAN. A comparative table showing equivalence of the considered criteria for morphological nomenclature of chromosomes, step by step instructions for LEVAN installation and examples can be found here.



- Abramoff, M.D., Magalhaes, P.J., Ram, S.J. "Image Processing with ImageJ". Biophotonics International, v.11, n.7, p.36-42, 2004.

- Green, D. M. and Sessions, S. K. Amphibian cytogenetics and evolution. In Appendix I, Nomenclature for chromosomes. Academic Press, Inc., USA, p.431-432, 1991.

- Levan, A., Fredga, K., Sandberg, A. A. Nomenclature for centromeric position on chromossomes. Hereditas, v.52, p.201-220, 1964.

- Rasband, W.S. ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA,, 1997-2012.

- Sakamoto, Y. and Zacaro, A. A. LEVAN, an ImageJ plugin for morphological cytogenetic analysis of mitotic and meiotic chromosomes. Initial version. An open source Java plugin distributed over the Internet from, 2009.


If you have questions, comments and/or would like to contribute with some cytogenetic data, please contact us:

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